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Immunoelectron Microscopy
Methods and Protocols
Series: Methods in Molecular Biology, Vol. 657
Schwartzbach, Steven D.; Osafune, Tetsuaki (Eds.)
1st Edition., 2010, XI, 352 p. 82 illus., 41 in color., Hardcover
ISBN: 978-1-60761-782-2

Due: July 29, 2010
152,01$
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About this book
Immunoelectron microscopy is a key technique that bridges the information
gap between biochemistry, molecular biology, and ultrastructural studies
placing macromolecular functions within a cellular context. In Immunoelectron
Microscopy: Methods and Protocols, expert researchers combine the
tools of the molecular biologist with those of the microscopist. From
the molecular biology toolbox, this volume presents methods for antigen
production by protein expression in bacterial cells, methods for epitope
tagged protein expression in plant and animal cells allowing protein
localization in the absence of protein specific antibodies as well
as methods for the production of anti-peptide, monoclonal, and polyclonal
antibodies. From the microscopy toolbox, sample preparation methods
for cells, plant, and animal tissue are presented. Both cryo-methods,
which have the advantage of retaining protein antigenicity at the
expense of ultrastructural integrity, as well as chemical fixation
methods that maintain structural integrity while sacrificing protein
antigenicity have been included, with chapters examining various aspects
of immunogold labeling. Written in the highly successful Methods in
Molecular Biology? series format, chapters include introductions to
their respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Authoritative and
essential, Immunoelectron Microscopy: Methods and Protocols seeks
to facilitate an increased understanding of structure function relationships.
Content Level " Professional/practitioner
Keywords " Fixation protocols - Macromolecular functions - Pre-
and post-embedding immunogold labeling - Structure function relationships
Related subjects " Cell Biology - Immunology |
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